A suspected salmonella outbreak at Melbourne’s five-star Langham hotel has left at least 44 people ill with six needing hospital treatment.

Victoria’s Department of Health is investigating the issue, which reportedly occurred after victims dined at the high tea room in the Aria Bar and Lounge between 11 and 12 July.

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The hotel is co-operating with the investigation and provided samples to the department for testing. However, the source of the salmonella outbreak has not been determined yet.

"As the safety and well-being of the guests and colleagues are of the highest priority, the hotel has extensive protocols in place to ensure food safety."

The Daily Mail cited a hotel spokeswoman saying that food samples were frozen in line with hotel guidelines.

The spokeswoman said: "This has never happened before and now we’re co-operating with the Department of Health and Human Services, and providing details of preparation logs and samples.

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"As the safety and well-being of the guests and colleagues are of the highest priority, the hotel has extensive protocols in place to ensure food safety."

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The latest salmonella case has emerged when Victoria is already dealing with escalating food poisoning cases with confirmed salmonellosis surging to 130% in the past six years.

In 2015, 2,124 salmonella cases have been identified. This is before the rise in temperature during the next few months when bacteria quickly multiply, reported Herald Sun.

The Langham hotel is located along Southbank Promenade at 1 Southgate Avenue. It boasts 338 rooms and suites overlooking the Yarra River.

Apart from the Aria Bar, the hotel also features Melaba restaurant and Langham restaurant. The Langham Melbourne features Chuan spa, a 5m indoor pool, along with ballrooms and conference facilities.